Genotoxic effects of panoramic radiation by assessing the frequency of micronuclei formation in exfoliated buccal epithelium

Authors

  • Vidya KB Department of Oral Pathology, College of Dental Science, Davangere, Karnataka
  • Anupama N. Kalappanavar Department of Pathology, Kasturba Medical College, Mangalore, Karnataka
  • Muniyappa M Department of Pathology, Shimoga institute of Medical Science, Shimoga, Karnataka

Keywords:

Panoramic radiography, Buccal epithelial cells, Genotoxic effects, Micronuclei

Abstract

Background:Panoramic radiography is one of the most commonly used radiographic methods to complement clinical examination. Ionizing radiation is a well-known mutagen and carcinogen in the human population. So this study was undertaken to evaluate the possible genotoxic effects of panoramic radiation by assessing the frequency of micronuclei formation in the exfoliated buccal epithelium.

Methods:50 patients of either sex in the age range of 15 to 75 years with apparently normal oral mucosa with no adverse habits and without any oral lesions were included in the present study after their consent. Buccal epithelial cells were obtained from the buccal mucosa by scraping with the toothbrush immediately before and after 10 ± 2 days of exposure to panoramic radiography. Cytological preparations were stained and observed under microscope. Student’s paired‘t’ test was used for the comparison between mean frequency of micronuclei in buccal epithelial cells in patients before and after panoramic radiography.

Results:Significant increase (P <0.0001) in the frequency of cells with micronuclei and total number of micronuclei after panoramic radiography was detected.  

Conclusion:The X-radiation emitted during panoramic radiography does induce some genotoxic changes in the form of increased frequency of micronuclei in target buccal epithelial cells.

 

References

Papova L, Kishilova D, Hadjidekova VB, Hristova RP, Atanasova VV et al. Micronucleus Test in Buccal Epithelium cells from patients subjected to Panoramic Radiography. Dentomaxillofac Radiol. 2007;36:168-71.

Riberio DA, De Oliveira G, De Castro GM, Angeilieri F. Cytogenetic biomonitoring in patients exposed to dental X-rays: comparison between adults and children. Dentomaxillofac Radiol. 2008;37:404-7.

Cerqueira EM, Meireles JRC, Lopes MA, Junquiera VC, Gomes-Filho IS et al. Genotoxic effects of X-rays on keratinized mucosa cells during panoramic dental radiography. Dentomaxillofac Radiol. 2008;37:398-403.

Maffei F, Angelini S, Forti GC, Lodi V, Violante FS et al. Micronuclei frequencies in hospital workers occupationally exposed to low levels of ionizing radiation: influence of smoking status and other factors. Mutagenesis. 2002;17(5):405-9.

Erdemir EO, Sengun A, Ulcer M. Cytotoxicity of mouthrinses on epithelial cells by micronucleus test. Eur J Dent. 2007;1:80-5.

Fenech M, Bolognesi C, Volders MK, Bonassi S, Zeiger E, et al. Harmonization of the micronucleus assay in human buccal cells: a Human Micronucleus (HUMN) project. Mutagenesis. 2007;22(1):3-4.

Norppa H, Falck GCM. What do human micronuclei contain? Mutagenesis. 2003;18(3):221-33

Pastor S, Gutierrez S, Creus A, Xamena N, Piperakis S et al. Cytogenetic analysis of Greek farmers using the micronucleus assay in peripheral lymphocytes and buccal cells. Mutagenesis. 2001;16(6):539-45.

Papova L, Hadjidekova V, Karadjov G, Agova S, Traskov D et al. Cytogenetic analysis of blood lymphocytes after arteriography (exposure to x-rays and contrast medium). Radiol Oncol. 2005;39(2):153-8.

Halder1 A, Chakraborty1 T, Mandal K., Gure PK, Das S et al. Comparative study of exfoliated oral mucosal cell micronuclei frequency in normal, precancerous and malignant epithelium. Int J Hum Genet. 2004;4(4):257-60.

Ozkul Y, Donmez H, Erenmemisoglu A, Demirtas H, Imamoglu N. Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users. Mutagenesis. 1997;12(4):285-7.

Brenner DJ, Doll R, Goodhead DT, Hall EJ, Land CE et al. Cancer risk attributable to low doses of ionizing radiation: assessment what we know. Proceedings N Acad Sci. 2003;24:13761-6.

Surralles J, Autio K, Jarventus H, Norppa H, Veidebaum T et al. Molecular cytogenetic analysis of buccal cells and lymphocytes from benzene exposed workers. Carcinogenesis. 1997;18(4):817-23.

Downloads

Published

2017-01-23

How to Cite

KB, V., Kalappanavar, A. N., & M, M. (2017). Genotoxic effects of panoramic radiation by assessing the frequency of micronuclei formation in exfoliated buccal epithelium. International Journal of Research in Medical Sciences, 2(2), 541–544. Retrieved from https://www.msjonline.org/index.php/ijrms/article/view/2194

Issue

Section

Original Research Articles