Effects of aloe vera gel application on epidermal wound healing in the domestic rabbit

Authors

  • Olaifa Abayomi Kayode Department of Veterinary Surgery and Reproduction, University of Ibadan, Ibadan, Nigeria

DOI:

https://doi.org/10.18203/2320-6012.ijrms20164531

Keywords:

Aloe vera gel, Contraction, Epidermal wounds, Haematology, Topical application

Abstract

Background: A wound is a breakdown in the protective function of the skin or loss of continuity of epithelium, with or without loss of underlying connective tissues, muscles, nerves, bones following injury to the skin, surgery, a blow, cut, chemicals, heat, cold, friction, shear force, pressure or diseases such as leg ulcers or carcinomas. A study was undertaken to determine the healing properties of Aloe vera gel on epidermal wounds in rabbits.

Methods: Twelve adult rabbits were divided into two groups randomly of six each representing the treatment and control respectively. A pair of wounds measuring 2cm x 2cm each was created on the back of each rabbit lateral to the spinal cord. The wounds were treated with homogenized Aloe vera gel while the wounds on control group were treated with normal saline. Wound contraction was measured on days 5, 9 and 12 representing the inflammatory, proliferative and maturation phases of wound healing respectively. Blood samples were collected on days 0, 3, 6, 9 and 12 for analysis.

Results: Animals treated with Aloe vera gel had significantly (p<0.05) faster rates of healing with shorter days of scab fall off than the control and showed significant (P<0.05) changes in the packed cell volume, mean corpuscular volume, lymphocyte and neutrophil counts.

Conclusions: The study concluded that Aloe vera was effective in treating epidermal wounds in rabbits over the control. An improvement occured in haematological profile of the experimental animals and these findings will go a long way in expanding the horizon of clinical application of this plant in solving wound healing problems in both humans and other animal species.

References

Leaper DJ, Harding KG. Wounds: Biology and Management. Oxford University Press. 2008.

Velnar T, Bailey T, Smrkolj V. The Wound Healing Process: an Overview of the Cellular and Molecular Mechanisms. J Int Med Res. 2009;37:1528-42.

Fernandez R, Griffiths R. Water for wound cleansing." Cochrane database of systematic reviews. 2015. CD003861. PMID 22336796.

Clinimed.co.uk. Wound Essentials: Phases of Wound Healing. Accessed on 30th June, 2015

Mercandetti M. Wound healing and repair. 2011. Emedicine.medscape.com

Yates A. Yates Garden Guide. Harper Collins (Australia) Pty Ltd, 466 pages. 2002.

Coats BC. The Silent Healer. A Modern Study of Aloe vera, Texas. Garland. 2008.

Surjushe A, Vasani R, Saple DG. Aloe vera: A Short Review. Indian Journal of Dermatology. 2008;53(4):163-6.

Foster M, Hunter D, Samman S. Evaluation of the nutritional and metabolic effects of Aloe vera. In: Benzie IFF, Wachtel-Galor, S. Eds. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd Edition. Boca Raton. 2011. Chapter 3.

Mukherjee PK, Nema NK, Maity N, Mukherjee K, Harwansh RK. Phytochemical and therapeutic profile of Aloe vera. J Nat Reme. 2014;14(1).

Reynolds T, Dweck AC. Aloe vera Leaf Gel: A Review Update. J. Ethnopharmacol. 2009;15:3-37.

Linnaeus, C. Systema Naturae, ed. 10, Holminae. Laurentius salvii. 1758:(1-4):1-824.

Cooper C. The complete beginner’s guide to Raising Small Animals: Everything you need to know about raising cows, sheep, chickens, ducks, rabbits and more. Atlantic Publishing Company. 2012:384.

Jain NC. Schalm’s Veterinary Haematology, 4th Edition (Ed. N. C. Jain), Lea and Febiger, Philadelphia. 1986:1221.

Dacie JV, Lewis SM. Practical Haematology, 6th Edition, Churchill, Livingstone, Edinburgh, Melbourne and New York. 1984;24-36.

Martin P. Wound Healing-aiming for perfect skin regeneration. Science. 2007;276:75-81.

Theoret CL. Physiology of Wound Healing. In: Equine wound Management, Second Edition (Eds. Stashak, T.S. and Theoret, C.L.), Veterinary Wound Management Society and Wiley-Blackwell, USA. 2008.

Davis RH, Leitner MG, Russo JM, Byrne ME. Wound Healing, Oral and Topical Activity of Aloe Vera. J Ame Podi Med Ass. 2009;79(11):P559-62.

Salcido R. Fruits, berries and nuts: phytochemicals in wound healing. Advances in skin and wound care. 2014:27(7):296.

Chithra R, Sajithlal GB, Chandrakasan G. Influence of aloe vera on collagen characteristics in healing dermal wounds in rats. Molecular and Cellular Biochemistry. 1998:181:71-6.

Heggers JP, Kucukcelebi A, Listengarten D, Stebenau J, Ko F, Broemeling LD, et al. Beneficial effect of aloe on wound healing in an excisionalwound model. J Alter and Comple Med. 1996;2:271-7.

Davis RH, Leitner MG, Russo JM, Byrne ME. Wound Healing, Oral and Topical Activity of Aloe Vera. J Amer Podi Med Ass. 2009;79(11):559-62.

Ogura T, Ueda H, Hosohara K, Tsuji R, Nagata Y, Kashiwamura S et al. Interleukin-18 stimulates hematopoietic cytokine and growth factor formation and augments circulating granulocytes in mice. The American Society of Hematology. Blood. 2001;98(7):2101-7.

Fibbe W-E, Willemze R. The role of interleukin-1in hematopoiesis. Acta Haematol. 1991;86:148-54.

Socolovsky M, Lodish HF, Daley GQ. Control of hematopoietic differentiation: Lack of specificity in signaling by cytokine receptors. Proc. Natl. Acad. Sci. USA. 1998;95:6573-5.

Luzina IG, Pickering EM, Kopach P, Kang PH, Lockatell V, Todd N, et al. Full-length IL-33 promotes inflammation but not Th2 response in vivo in an ST2-independent fashion. J Immunol. 2012;189:403-10.

Gillery P, Fertin C, Nicolas JF, Chastang F, Kalis B, Banchereau J, et al. Interleukin-4 stimulates collagen gene expression in human fibroblast monolayer cultures. Potential role in fibrosis. FEBS Lett. 1992;302:231-4.

Postlethwaite AE, Holness MA, Katai H, Raghow R. Human fibroblasts synthesize elevated levels of extracellular matrix proteins in response to Interleukin 4. J Clin Invest. 1992;90:1479-85.

Devalaraja RM, Nanney LB, Du J, Qian Q, Yu Y, Devalaraja MN, et al. Delayed wound healing in CXCR2 knockout mice. J. Invest. Dermatol. 2000;115:234-44.

Ashcroft GS, Lei K, Jin W, Longnecker G, Kulkarni AB, Greenwell-Wild, T, et al. Secretory leukocyte protease inhibitor mediates non-redundant functions necessary for normal wound healing. Nat. Med. 2000;6:1147-53.

Dovi JV, Li-Ke H, DiPietro LA. Accelerated wound closure in neutrophil-depleted mice J. Leukoc. Biol. 2003;73:448-55.

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Published

2016-12-19

How to Cite

Kayode, O. A. (2016). Effects of aloe vera gel application on epidermal wound healing in the domestic rabbit. International Journal of Research in Medical Sciences, 5(1), 101–105. https://doi.org/10.18203/2320-6012.ijrms20164531

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Original Research Articles