Serum adenosine deaminase as oxidative stress marker in type 2 diabetes mellitus
Keywords:Serum adenosine deaminase, Type 2 diabetes mellitus, MDA
Background: Oxidative stress markers are increased in type 2 diabetes mellitus and its estimation helps in predicting the long term complications. In present study comparison and correlation of the levels of serum adenosine deaminase, serum malondialdehyde, and serum total antioxidant capacity in type 2 diabetes mellitus and in age and sex matched healthy controls.
Methods: Study group consisted of 100 individuals between the age group of 35-65 years of age. Of which 50 individuals with type 2 diabetes mellitus were considered as cases. The control group consisted of 50 age and sex matched healthy individuals. Study was approved by institutional ethical committee. By aseptic precautions 2 ml of venous blood was collected in a plain vacutainer tube, after 8-12 hours of fasting. Serum adenosine deaminase, serum malondialdehyde, and serum total antioxidant capacity were estimated in all groups.
Results: The study observed an increased level of serum adenosine deaminase, malondialdehyde and decreased levels of total antioxidant capacity in type 2 diabetes mellitus compared to controls. Serum adenosine deaminase levels in type 2 diabetics were 50.77 ± 6.95 and in controls was 17.86 ± 4.04. Serum Malondialdehyde levels in type 2 diabetics was 512.13 ± 70.15 and in controls was 239.32 ± 23.97. Serum total antioxidant levels in type 2 diabetics was 0.39±0.15 and in controls was 1.66±0.25. Positive correlation was seen between serum adenosine deaminase and malondialdehyde and it was statistically significant. Statistically significant negative correlation was seen between serum adenosine deaminase and total antioxidant capacity.
Conclusion: Adenosine deaminase can be used as oxidative stress marker. Their increased levels indicate oxidative stress in type 2 diabetes mellitus. Therefore, estimation of serum adenosine deaminase levels help in early prediction and prevention of long term complications occurring due to oxidative stress in diabetics, thereby decreasing the mortality and morbidity in them.
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