Effect of cyclophosphamide on the microanatomy of liver of albino rats

Authors

  • Javed Ahmad Khan Assistant Professor, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Shaheen Shahdad Professor, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Mohd. Arif Makhdoomi Demonstrator, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Sajad Hamid Lecturer, Department of Anatomy, SKIMS Medical College, Bemina, J&K
  • Gh. Muhammad Bhat Associate Professor, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Yasmeen Jan Assistant Professor, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Sabia Nazir Assistant Professor, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Zahid Bashir Lecturer, Department of Anatomy, Govt. Medical College, Srinagar, J&K
  • Shameema Banoo Lecturer, Department of Anatomy, Govt. Medical College, Srinagar, J&K

Keywords:

Cyclophosphamide, Fatty infiltration, Central vein congestion

Abstract

Background: Cyclophosphamide is widely used in the treatment of various neoplastic diseases and diseases associated with altered immunity. Higher doses used for longer duration effects many organs like urinary bladder, lungs, liver, heart and male reproductive organs.

Methods: To study the effect of cyclophosphamide on the micro anatomy of liver, sixty eight Albino rats were taken and divided into three groups, group A (control group) of 20 animals, were fed with routine diet, group B (low dose group) of 24 animals, were given cyclophosphamide at the dose of 0.5 mg/100 gms in addition to the routine diet and group C (high dose group) of 24 animals, were given high dose of cyclophosphamide at the dose of 0.7 mg/100 gms of weight of animal in addition to the routine diet. The animals were sacrificed at intervals of 3, 6, 9 and 12 weeks, 5 microns sections of the tissue were prepared and stained with Haematoxylin and Eosin stain.

Results: Microscopic changes in liver were apparent in the drug treated animals. In group B the changes appeared after 6 weeks while in group C they started appearing after 3 weeks of drug treatment. The changes were in the form of fatty changes, hemorrhages and central vein congestion.

Conclusion: Cyclophosphamide induces histological changes like fatty infiltration and central vein congestion in the liver. These changes are with low doses given for longer durations and manifest earlier when larger doses
are used. Thus it is advised that patients receiving cyclophosphamide should be periodically evaluated for liver dysfunction.

 

References

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Published

2017-01-26

How to Cite

Khan, J. A., Shahdad, S., Makhdoomi, M. A., Hamid, S., Bhat, G. M., Jan, Y., Nazir, S., Bashir, Z., & Banoo, S. (2017). Effect of cyclophosphamide on the microanatomy of liver of albino rats. International Journal of Research in Medical Sciences, 2(4), 1466–1469. Retrieved from https://www.msjonline.org/index.php/ijrms/article/view/2442

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Original Research Articles