Study of antioxidant status in malaria patients

Authors

  • Amit G. Tyagi Department of Biochemistry, GMERS, Medical College, Junagarh, Gujarat, India
  • Rupal A. Tyagi Department of Biochemistry, GMERS, Medical College, Junagarh, Gujarat, India
  • Prema Ram Choudhary Department of Physiology, C.U. Shah Medical College, Surendranagar, Gujarat, India
  • Jaidev Singh Shekhawat Department of Anatomy, C.U. Shah Medical College, Surendranagar, Gujarat, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20171281

Keywords:

Glutathione peroxidase, Malondialdehyde, Malaria, Plasmodium falciparum, Superoxide dismutase

Abstract

Background: Oxidative stress plays an important role in the development of metabolic changes in malaria patients. During infection RBCs are exposed to continual oxidative stress. The univalent reduction of oxygen results in a series of cytotoxic oxygen species such as O2-, H2O2, OH•. Objective was to determine the level of oxidative stress in patients suffering from malaria.

Methods: The present study was conducted on 551 malaria patients and 211 age-sex matched controls, in department of Biochemistry, C U Shah Medical College, Surendranagar, Gujarat from April 2012 to May 2013.  In stage-I, day-1 malaria patient’s v/s control group, In stage-II, day-3 v/s day-1 after anti-malarial treatment and in Stage-III day-3 v/s day-1 after anti-malarial + antioxidant treatment.

Results: The mean erythrocytic activity of SOD, CAT, GST were decreased (0.71±0.25EU, 9.9±2.4μmol/sec, and 11.7±3.9 U/gmHb% respectively), mean level of GSH and MDA were increased (42.1±6.06gm/Hb%, 10.9±2.83 respectively) significantly (P<0.001) as compared to control group. In the follow up study with anti-malarial treatment the mean levels of erythrocytic GSH and MDA (28.7±7.54gm/Hb% and 8.08±1.95nM/L) decreased significantly (P<0.001 and P<0.01 respectively), whereas mean activity of erythrocytic enzymes like SOD, CAT and GST (0.99±0.15 EU, 15.8±2.68μmol/sec and 22.5±5U/gmHb%) were increased significantly (P<0.001) as compared to day-1.

Conclusions: Erythrocytic antioxidant enzymes, GSH and MDA may be considered to be reliable biochemical markers for diagnostic and therapeutic potential in malaria.

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Published

2017-03-28

How to Cite

Tyagi, A. G., Tyagi, R. A., Choudhary, P. R., & Shekhawat, J. S. (2017). Study of antioxidant status in malaria patients. International Journal of Research in Medical Sciences, 5(4), 1649–1654. https://doi.org/10.18203/2320-6012.ijrms20171281

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Original Research Articles