Microbial spectrum of urinary tract infections and its antibiogram in a tertiary care hospital

Authors

  • Monika Yadav Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India
  • Rohan Pal Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India
  • Shan Damrolien Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India
  • Sulochana D. Khumanthem Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20172476

Keywords:

Antibiotic policies, Antibiotic susceptibility, Rational choice, Urinary tract infections

Abstract

Background: Urinary tract infections are one of the major health problem effecting both sexes of all age group. UTIs are often treated with different broad-spectrum antibiotics. The aim of this study was to determine the prevalence of bacteria causing urinary tract infections and their susceptibility pattern from patients reporting in RIMS Hospital.

Methods: Mid stream urine (MSU) specimens sent to the laboratory from October 2014 to September 2016 were collected and inoculated onto blood agar and MacConkey agar and incubated at 37ºC for 24 hours. Identification and antibiotic susceptibility test was done following standard operative procedures.

Results: 25.66% (1142/4450) samples showed a significant growth out of which 42% (479/1142) were male and 58% (663/1142) were female. E. coli has been found to be the major pathogen causing UTI which account for 61% (696/1142) followed by Staphylococcus aureus 12% (137/1142), CONS 7% (79), Enterococcus spp. 6% (67), Klebsiella spp. 5% (57), Proteus spp. 2% (22), Pseudomonas spp. 2%, Acinetobacter spp. 2% and Candida spp. 3%. Imipenem was the most susceptible antibiotic for Enterobacteriaceae, E. coli (85.9%), Klebsiella spp. (89.4%) and Proteus spp. (95.4%). Vancomycin is 100% sensitive while Linezolid, Nitrofurantoin and Gentamicin are also highly sensitive for both Staphylococcus aureus and CONS.

Conclusions: These data may be used to determine trends in antimicrobial susceptibilities, to formulate local antibiotic policies in order to assist clinicians in the rational choice of antibiotic therapy to prevent misuse, or overuse, of antibiotics.

Author Biography

Monika Yadav, Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India

Post graduate trainee, Department of Microbiology.

References

Karlowsky JA, Kelly LJ, Thornsberry C, Jones ME, Sahm DF. Trends in antimicrobial resistance among urinary tract infection isolates of Escherichia coli from female outpatients in the United States. Antimicrob Agents Chemotherap. 2002;46(8):2540-5.

Shigemura K, Arakawa S, Sakai Y, Kinoshita S, Tanaka K, Fujisawa M. Complicated urinary tract infection caused by Pseudomonas aeruginosa in a single institution (1999-2003). Int J Urol. 2006;13(5):538-42.

Kolawole AS, Kolawole OM, Kandaki-Olukemi YT, Babatunde SK, Durowade KA, Kolawole CF. Prevalence of urinary tract infections (UTI) among patients attending Dalhatu Araf Specialist Hospital, Lafia, Nasarawa state, Nigeria. Int J Med Medi Sci. 2010;1(5):163-7.

Farrell DJ, Morrissey I, De Rubeis D, Robbins M, Felmingham D. A UK multicentre study of the antimicrobial susceptibility of bacterial pathogens causing urinary tract infection. J Infect. 2003;46(2):94-100.

Fish DN. Urinary tract infections. In: Kimble MAK, Young LY, Kradjan WA, et al, eds. Applied therapeutics: the clinical use of drugs. 9th ed. Philadelphia, PA: Lippincott, 2009.

Grabe M, Bishop MC, Bjerklund-Johansen TE, Botto H, Cek M, Lobel B, et al. Guideline on the management of urinary and male genital tract infections. Eur Assoc Urol. Update. 2008:8-106.

Kripke CL. Duration of therapy for women with uncomplicated UTI. Am Fam Physic. 2005;72(11):2219.

Kahlmeter G. An international survey of the antimicrobial susceptibility of pathogens from uncomplicated urinary tract infections: the ECO· SENS Project. J Antimicrob Chemother. 2003;51(1):69-76.

Collee JG, Miles RS, Watt B. Tests for the identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, eds. Mackie and McCartney Practical Medical Microbiology. 14th edition. India: Elsevier; 2007:131-148.

Clinical and Laboratory Standard Institute guideline: Performance standard for antimicrobial susceptibility testing: Wayne, PA-17 the informational supplement; 2007:M100-S17.

Garg N, Shukla I, Rizvi M, Ahmed SM, Khatoon A, Khan F. Microbiological profile and antibiotic sensitivity pattern of bacterial isolates causing urinary tract infection in intensive care unit patients in a tertiary care hospital in Aligarh region, India. Int J Curr Microbiol Applied Sci. 2015;1:163-72.

Jones RN. Impact of changing pathogens and antimicrobial susceptibility patterns in the treatment of serious infections in hospitalized patients. Am J med. 1996;100(6):3S-12S.

Beyene G, Tsegaye W. Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in Jimma university specialized hospital, southwest Ethiopia. Ethiopian J Health Sci. 2011;21(2):141-6.

Niranjan V, Malini A. Antimicrobial resistance pattern in Escherichia coli causing urinary tract infection among inpatients. Indian J Med Res. 2014;139(6):945.

Patel S, Taviad PP, Sinha M, Javadekar TB, Chaudhari VP. Urinary tract infections (UTI) among patients at GG hospital and medical college, jamnagar. Nat J Comm Med. 2012;3(1):138-41.

Miller O, Hemphill RR. UNINARY TRACT INFECTION AND PYELONEPHRITIS. Emergency medicine clinics of North America. 2001 Aug 1;19(3):655-74.

Foxman B. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. The American journal of medicine. 2002;113(1):5-13.

Hasan AS, Nair D, Kaur J, Baweja G, Deb M, Aggarwal P. Resistance patterns of urinary isolates in a tertiary Indian hospital. J Ayub Med Coll Abbottabad. 2007;19(1):39-41.

Savitha T, Murugan K, Thangamariappan K. Prevalence study on emergence of urinary tract infection in Erode, Tamilnadu, India. Int J Curr Res. 2011;2(1):5-13.

Sood S, Gupta R. Antibiotic resistance pattern of community acquired uropathogens at a tertiary care hospital in Jaipur, Rajasthan. Indian J Comm Med. 2012;37(1):39.

Sasirekha B. Prevalence of ESBL, AmpC β-lactamases and MRSA among uropathogens and its antibiogram. 2013.

Khameneh ZR, Afshar AT. Antimicrobial susceptibility pattern of urinary tract pathogens. Saudi J Kidney Dis Transplant. 2009;20(2):251.

Rajesh KR, Mathavi S, Priyadarsini RI. Prevalence of antimicrobial resistance in uropathogens and determining empirical therapy for urinary tract infections. International Journal. 2010 Oct;1(5):260.

Babypadmini S, Appalaraju B. Extended spectrum-lactamases in urinary isolates of Escherichia coli and Klebsiella pneumoniae-prevalence and susceptibility pattern in a tertiary care hospital. Indian J Med Microbiol. 2004;22(3):172.

Poovendran P, Vidhya N, Murugan S. Antimicrobial susceptibility pattern of ESBL and non-ESBL producing uropathogenic Escherichia coli (UPEC) and their correlation with biofilm formation. Int J Microbiol Res. 2013;4(1):56-63.

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Published

2017-05-27

How to Cite

Yadav, M., Pal, R., Damrolien, S., & Khumanthem, S. D. (2017). Microbial spectrum of urinary tract infections and its antibiogram in a tertiary care hospital. International Journal of Research in Medical Sciences, 5(6), 2718–2722. https://doi.org/10.18203/2320-6012.ijrms20172476

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Original Research Articles