Bacterial flora in the conjunctiva among the patients undergoing cataract surgery

Authors

  • K. Satish Department of Ophthalmology, GSL Medical College, Rajahmundry, Andhra Pradesh, India
  • T. Jaya Chandra Department of Microbiology, GSL Medical College, Rajahmundry, Andhra Pradesh, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20191326

Keywords:

Bacteria, Bacilli, Cocci, Conjunctiva, Flora, Surgery

Abstract

Background: The present study was aimed in identifying the normal organisms found in the conjunctiva of healthy eyes among the individuals undergoing cataract surgery and also antibacterial susceptibility of the isolates. Cataract is the major cause of blindness in the developing countries, cataract surgery is one of the common surgical practices.

Methods: Study was conducted in the department of Microbiology, GSL Medical College from May to July 2018. Surgically removed cataract lens was collected in sterile nutrient broth and transported to Microbiology laboratory for culture and sensitivity testing. The broths were incubated at 37ºC for 24 hours subcultured on Blood agar, MacConkey agar and Nutrient agar, incubated aerobically at 37ºC for 24 hours and then examined for bacteria growth according to standard protocol. After incubation growth was identified by colony morphology, gram staining and biochemical tests. Antibiotic sensitivity testing was done on Muller-Hinton agar by the disk diffusion method.

Results: During the study period, 58 samples were collected; the male female ratio was 1.14. Maximum strains were isolated among female; the male female ration was 0.95. Increase in flora was observed with age. No significant drug resistance was observed.

Conclusions: This is a hospital based study which is not true representation of the community. Hence a community based study with large sample by considering the comorbid conditions such as occupation, diabetes, immune status can give the authentic data on the conjunctival flora.

References

Murthy GVS, Gupta SK, John N, Vashist P. Current status of cataract blindness and vision 2020: The right to sight initiative in India. Indian J Ophthalmol. 2008;56:489494.

Lin YH, Kang YC, Hou CH, Chiun-Ho Hou, Yhu-Chering H, Chih-Jung C, et al. Antibiotic susceptibility profiles of ocular and nasal flora in patients undergoing cataract surgery in Taiwan: an observational and cross-sectional study. BMJ Open. 2017;7:e017352.

Dass SP, Sharma N, Gupta RK, Singh P. Aerobic bacterial flora of the normal conjunctiva at high altitude area of Shimla Hills in India: a hospital based study. Int J Optholmol. 2013;6(5):723-6.

Jaya Chandra T, Sowndarya AJ, Sirisha I, Sharma YV. How Safe Ladies Hand Bags Are: A Microbiological View. Biol and Med. 2014;6:3:1000212.

Jayachandra T, LakshmiprasannaT VA. Astudy on isolation and identificationofbacteria causing nosocomial infections on mobile phones of health care workers. Calicut Med J. 2011;9(1):e2.

Mackie and Mac Cartney Practical medical Microbiology: J.G. Collee, A.G. Fraser, B.P. Marnion, A. Simmons Editors. Tests for the identification of bacteria. 14th Ed., Churchill living stone publisher; 1999:131-150.

Bailey and Scott’s Diagnostic Microbiology, Karan Fabiano Editor. Laboratory cultivation and isolation of bacteria, methods for bacterial identification. 11th Ed., Andrew Allen Publisher; 2002:133-167.

Rajvanshi VS. Bacterial flora of the conjunctiva. J All India Ophthalmol Soc. 1968;16:24-8.

Khorazo D, Thompson R. The bacterial flora of the normal conjunctiva. Am J Opthalmol. 1935;18:1114-6.

Ansari MR, Madani H, Ghaderi E. Conjunctival bacterial flora and antibiotic resistance pattern in patients undergoing cataract surgery. Pak J Med Sci. 2008;24:581-5.

Chang HL. Bacterial flora of the normal conjunctiva. Chin Med J. 1957;75:233-5.

Matuura H. Anaerobes in the bacterial flora of the conjunctival sac. Jpn J Opthol. 1971;15:116-24.

Locatcher- Khoranzo D, Seegal BC. Microbiology of the eye. St. Louis: Mosby. 1972:13-8.

Starr MB, Lally JM. Antimicrobial prophylaxis for ophthalmic surgery. Surv Opthamlol. 1995:39:485-501.

Bachrach U, Gurevitch J, Landau J, Birnbaum D. The flora of the normal conjunctiva of healthy people in Israel. Acta Med Orient. 1953;12:10-3.

Debnath Sc. Effect of preoperative antibiotics on bacterial colony counts of the conjunctival sac. J Ocul Ther Surg. 1985;4:122-4.

Smith CH. Bacteriology of the healthy conjunctiva. Br J Ophthalmol. 1954;38:719-26.

Agarwal LP, Khosla PK. Mycotic keratitis experimental and Clinical study. All India Opthmol Soc. 1967;15:1-10.

Rao PN, Rao KN. Study of the normal conjunctival flora (bacterial and fungal) and its relations to external ocular infections. Indian J Ophthalmol. 1972;20:164-70.

Javed EA, Sultan M, Mehmood RQ. Normal conjunctival flora. Growth. 2008;78:15-6.

Kudva AA, Kadri R, Bhandary R, Hegde KS, Achar A, Devika P, et al. A study of the normal bacterial flora on the conjunctiva of patients undergoing cataract surgery to select the best pre-operative topical antibiotic. Int J A J Insti Med Scienc. 2012;1:139-42.

Liu J, Li J, Huo J, Xie HP. Identification and quantitation of conjunctival aerobic bacterial flora from healthy residents at different ages in Southwest China. African J Microbiolo Res. 2011;5:192-7.

Singer TR, Isenberg SJ, Apt L. Conjunctival anaerobic and aerobic bacterial flora in paediatric versus adult subjects. Br J Ophthalmol. 1988;72:448-51.

Sinha BN, Das MS. Bacterial and fungal flora of the conjunctiva in Healthy and Diseased eyes. Ind J Med Assoc. 1968;51(5):217.

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Published

2019-03-27

How to Cite

Satish, K., & Chandra, T. J. (2019). Bacterial flora in the conjunctiva among the patients undergoing cataract surgery. International Journal of Research in Medical Sciences, 7(4), 1208–1211. https://doi.org/10.18203/2320-6012.ijrms20191326

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Original Research Articles