Screening for microbial load and antibiotic resistance pattern in Escherichia coli isolated from paper currency circulating in Kushtia, Bangladesh

Authors

  • S. M. Raihan Rahman Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, Bangladesh
  • Md. Nazim Uddin Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, Bangladesh
  • Zulkar Nain Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, Bangladesh
  • Mohammad Minnatul Karim Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia, Bangladesh

DOI:

https://doi.org/10.18203/2320-6012.ijrms20191318

Keywords:

Antibiotic resistance, Escherichia coli, Microbial load, Paper currency

Abstract

Background: Paper currency is used for every type of commerce and plays an important role in the life of human beings. They are exchanged and come into contact with different environments and many different individuals during their circulation. Therefore, they can become contaminated with microorganisms and transfer bacteria across environments. The present study was aimed for quantitative assessment of microorganisms in circulated paper currency from Kushtia, Bangladesh and antibiotic resistant profiles of isolated Escherichia coli.

Methods: A total of 10 paper currency samples currently in circulation involving three denominations (5, 10 and 500) were randomly collected from individuals involved in various occupations including street beggar, local hotel, bus conductor, poultry seller, vegetable seller, fish seller, commercial bank, ATM booth, tea seller, grocery store in Kushtia city, Bangladesh. Selective culture and biochemical tests were performed for the isolation and identification of microbial pathogens. Antibiotic resistance profiles were evaluated for isolated Escherichia coli using Kirby-Bauer method according to CLSI guidelines.

Results: Aerobic mesophilic bacteria, Enterobacteriaceae and Pseudomonas spp. were the highest in paper currency from local hotel and ATM booth. Enterobacteriaceae (including coliforms) were predominantly present in paper currencies collected from local hotel, grocery, fish seller and beggar while Pseudomonas spp. were found in currency notes obtained from ATM booth, poultry farm, vegetable seller and local hotel. Antibiotic resistant profiles of E. coli isolated from local hotel currency showed that 50% of E. coli isolates were multidrug resistant. The highest resistant profile was observed against penicillin (95%) followed by polypeptide (75%), cephalosporin (50%), quinolone (30%) and sulfonamide (5%) groups of antibiotics.

Conclusions: Multiple antibiotic resistant pathogenic bacteria are prevalent in paper currency regardless of their sources. Paper currency could contribute in transmission of infectious disease as well as in antibiotic resistance, therefore, should be handled carefully.

References

Girma G, Ketema T, Bacha K. Microbial load and safety of paper currencies from some food vendors in Jimma Town, Southwest Ethiopia. BMC Res Notes. 2014;7(1):843.

Moses IB, Ugbo EN, Iroha IR, Ukpai EG, Odah EE. Antibiogram and phenotypic characterization of E. coli isolated from Nigeria’s paper currencies obtained from butchers in Ebonyi state. Arch Clin Microbiol. 2018;9(4):85.

Boidya J, Uddin R, Mandal SC. Microbiological analysis of Bangladeshi paper currency circulating in Dhaka city. Biores Comm. 2015;1(1):53-6.

Sharif M, Ansari F. Evaluation of health risk from handling Pakistani currency notes and coins in Lahore. World J Pharm Res. 2017;6 (6):129-59.

Borah D, Parida P, Kumar T. Paper currencies, a potential carrier of pathogenic microorganisms. Int J Applied Biol Pharmaceut Tech. 2012;3(1):23-5.

Abrams BL, Waterman NG. Dirty money. JAMA. 1972;219(9):1202-3.

Nolte O. Antimicrobial resistance in the 21st century: a multifaceted challenge. Protein Peptide Letters. 2014;21(4):330-5.

Economou V, Gousia P. Agriculture and food animals as a source of antimicrobial-resistant bacteria. Inf Drug Res. 2015;8:49.

Golkar Z, Bagasra O, Pace DG. Bacteriophage therapy: a potential solution for the antibiotic resistance crisis. J Inf Dev Countries. 2014;8(02):129-36.

Gould IM, Bal AM. New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence. 2013;4(2):185-91.

Ahmed MS, Parveen S, Nasreen T, Feroza B. Evaluation of the microbial contamination of Bangladesh paper currency notes (Taka) in circulation. Adv Biol Res. 2010;4(5):266-71.

Benson, HJ, Brown AE, eds. Benson’s Microbiological Applications: Microbiology. 10th ed. McGraw-Hill Higher Education; 2006.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4):493-6.

Ercumen A, Pickering AJ, Kwong LH, Arnold BF, Parvez SM, Alam M, et al. Animal feces contribute to domestic fecal contamination: evidence from E. coli measured in water, hands, food, flies, and soil in Bangladesh. Env Sci Tech. 2017;51(15):8725-34.

Umeh EU, Juluku JU, Ichor T. Microbial contamination of Naira (Nigerian Currency) notes in circulation. Res J Env Sci. 2007;1(6):336-9.

Anthony A, Anthony I. The in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus from hospital currency notes. Afr J Microbiol Res. 2011;5(14):1881-7.

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Published

2019-03-27

How to Cite

Rahman, S. M. R., Uddin, M. N., Nain, Z., & Karim, M. M. (2019). Screening for microbial load and antibiotic resistance pattern in Escherichia coli isolated from paper currency circulating in Kushtia, Bangladesh. International Journal of Research in Medical Sciences, 7(4), 1161–1165. https://doi.org/10.18203/2320-6012.ijrms20191318

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Section

Original Research Articles