Expression of MUC5AC in normal gastric mucosa, intestinal metaplasia and gastric carcinoma by immunohistochemistry

Authors

  • Shuaeb Bhat Department of Pathology, Government Medical College, Anantnag, Jammu and Kashmir, India
  • Nusrat Bashir Department of Pathology, Government Medical College, Srinagar, Jammu and Kashmir, India
  • Showkat Ahmad Mir Department of Pathology, Government Medical College, Anantnag, Jammu and Kashmir, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20191340

Keywords:

Gastric cancer, Immunohistochemistry, Intestinal metaplasia, MUC5AC

Abstract

Background: Carcinomas of the stomach are a heterogeneous group of lesions in terms of architecture, pattern of growth, cell differentiation, and histogenesis. Altered MUC5AC expression patterns have been reported previously in intestinal metaplasia as well as in gastric cancer. The aim of the study was to analyse the expression pattern of MUC5AC in normal, pre-neoplastic and neoplastic gastric epithelium.

Methods: Formalin fixed paraffin embedded sections of sixty cases which include twenty cases of each normal gastric mucosa, intestinal metaplasia and gastric carcinoma were taken up for the study and subjected to immunohistochemistry using MUC5AC.

Results: The intensity of MUC5AC immunostaining in normal gastric mucosa, intestinal metaplasia and gastric carcinoma was evaluated. Immunoreactivity was graded as 0 (negative), ± (trace positive), + (positive) or ++ (strongly positive). Statistical analysis was performed with Chi-Square test and significant differences were noted between these 3 groups (p value <0.05).

Conclusions: Authors concluded that MUC5AC expression rates might be good parameters in progression of intestinal metaplasia to gastric carcinoma and might be a good prognostic marker for gastric carcinoma as it is very well implicated in understanding of gastric carcinogenesis.

References

Kim YS, Gum J, Brockhausen I. Mucin glycoproteins in neoplasia. Glycoconj J. 1996;13:693-707.

Itoh Y, Kamata-Sakurai M, Denda-Nagai K, Nagai S, Tsuiji M, Ishii-Schrade K, et al. Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin. Glycobiol. 2008;18:74-83.

Andrianifahanana M, Moniaux N, Batra SK. Regulation of mucin expression: mechanistic aspects and implications for cancer and inflammatory diseases. Biochim Biophys Act. 2006;1765:189-222.

Kanoh A, Takeuchi H, Kato K, Waki M, Usami K, Irimura T. Interleukin-4 induces specific pp-GalNAc-T expression and alterations in mucin O-glycosylation in colonic epithelial cells. Biochim Biophys Acta. 2008;1780:577-584.

Byrd JC, Bresalier RS. Mucins and mucin binding proteins in colorectal cancer. Cancer Metastasis Rev. 2004;23:77-99.

Correa P. A human model of gastric carcinogenesis. Cancer Res. 1988;48:3554-60.

Rokkas T, Filipe M, Sladen G. Detection of an increased incidence of early gastric cancer in patients with intestinal metaplasia type III who are closely followed up. Gut. 1991;32:1110-13.

Nakasaki H, Mitomi T, Noto T. Mosaicism in the expression of tumor-associated carbohydrate antigens in human colonie and gastric cancers. Cancer Res. 1989;49:3662-69.

Kuan SF, Byrd JC, Basbaum CB, Kim YS. Characterization of quantitative mucin variants from a human colon cancer cell line. Cancer Res.1987;47: 5715-24.

Ho SB, Shekels LL, Toribara NW. Mucin gene expression in normal, pre-neoplastic, and neoplastic human gastric epithelium. Cancer Res. 1995;55: 2681-90.

Reis CA, David L, Nielsen PA, Clausen H, Mirgorodskaya K, Roepstorff P, Sobrinho‐Simões M. Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody. Int J Cancer. 1997;74(1):112-21.

Carneiro F, Amado M, David L, Clausen H, Sobrinho-Simões M. Glycosylation features of gastric carcinoma initiation and progression-a review with emphasis on simple mucin-type carbohydrates and histo-blood group antigens of the Lewis system. Eur J Cancer Prev. 1994;3:39-46.

Reis CA, David L, Correa

P. Intestinal metaplasia of human stomach displays distinct patterns of mucin (MUC1, MUC2, MUC5AC, and MUC6) expression. Cancer Res. 1999;59;1003.

Filipe MI, Jass JR. Intestinal metaplasia subtypes and cancer risk. Gastric Carcinoma. Churchill Livingstone: London; 1986: 87-115.

Ho SB, Roberton AM, Shekels LL. Lyftogt CT. Niehans GA, Toribara NW. Mucins in human gastric epithelium: expression cloning of gastric mucin cDNA and localization of mucin gene expression. Gastroenterol. 1995.

Reis CA, David L, Correa P, Carneiro F, Bolós C. Intestinal metaplasia of human stomach displays distinct patterns of mucin (MUC1, MUC2, MUC5AC, and MUC6) expression. Cancer Res. 1999:59(5):1003-7.

Jass JR, Filipe MI. The mucin profiles of normal gastric mucosa, intestinal metaplasia and its variants and gastric carcinoma. Histochem J. 1981;73:931-939.

Segura DI, Montero C. Histochemical characterization of different types of intestinal metaplasia in gastric mucosa. Cancer. 1983;52:498-503.

Silva, S, Filipe MI. Intestinal metaplasia and its variants in the gastric mucosa of Portuguese subjects: a comparative analysis of biopsy and gastrectomy material. Hum Pathol. 1986;17:988-995.

Lauren P. The two histological main types of gastric carcinoma: diffuse and so-called intestinal type carcinoma. Acta Pathol Microbiol Scand. 1965;64: 31-49.

Reis CA, David L, Carvalho F, Mandel U. Immunohistochemical study of the expression of MUC6 mucin and co-expression of other secreted mucins (MUC5AC and MUC2) in human gastric carcinomas. J Histochem Cytochem. 2000;48:377.

Roessler K, Mönig SP, Schneide PM, Hanisch FG. Co-expression of CDX2 and MUC2 in gastric carcinomas: correlations with clinico-pathological parameters and prognosis. World J Gastroenterol. 2005;11(21):3182-3188.

Ilhan O, Han U, Onal B, Celik S. Prognostic significance of MUC1, MUC2 and MUC5AC expressions in gastric carcinoma. Turk J Gastroenterol. 2010;21(4):345-352.

Borrmann R. Tumors of the stomach and duodenum. Digestive Tube. 1926; 4; 812-1054.

Ming SC. Gastric Carcinoma: a pathological classification. Cancer. 1977;39: 2475-2485.

Zhang HK, Zhang QM, Zhao TH, Li YY, Yi YF. Expression of mucins and E-cadherin in gastric carcinoma and their clinical significance. J Gastroenterol. 2004;10(20):3044-3047.

Babu SD, Jayanthi V, Devaraj N, Reis CA, Devaraj H. Expression profile of mucins (MUC2, MUC5AC and MUC6) in Helicobacter pylori infected pre-neoplastic and neoplastic human gastric epithelium. Mol Cancer. 2006;5:1-7.

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Published

2019-03-27

How to Cite

Bhat, S., Bashir, N., & Mir, S. A. (2019). Expression of MUC5AC in normal gastric mucosa, intestinal metaplasia and gastric carcinoma by immunohistochemistry. International Journal of Research in Medical Sciences, 7(4), 1282–1287. https://doi.org/10.18203/2320-6012.ijrms20191340

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Original Research Articles