Hepatoprotective and antiproliferative activity of moringinine, chlorogenic acid and quercetin

Authors

  • Fahmy T. Ali Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo
  • Nahla S. Hassan Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo
  • Rehab R. Abdrabou Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo

DOI:

https://doi.org/10.18203/2320-6012.ijrms20160799

Keywords:

Moringa oleifera, Quercetin, Moringinine, Chlorogenic acid, Antitumor effect

Abstract

Background: The three well-characterized phytochemicals in Moringa oleifera leaves are moringinine, quercetin, and chlorogenic acid. Moringa oleifera is postulated to have the highest antioxidant content in food and also has a remarkable range of medicinal uses and high nutritional value.

Methods: Evaluation of the antiproliferative effect of moringinine, quercetin and chlorogenic acid as well Moringa oleifera leaves extract on two human cell lines, breast cancer cell line (MCF7) and liver carcinoma cell line (HepG2) using the sulforhodamine B (SRB) assay. The efficacy on liver toxicity induced in rat by alloxan was carried out.

Results: Our results indicated that both quercetin and moringinine are strong antitumor agents against the two human cell lines, the extract and its three active ingredients improved the induced liver toxicity.

Conclusions: Quercetin and moringinine are responsible to a great extent for the antitumor activity of the whole extract. Chlorogenic acid is a potent hepatoprotective in alloxan induced liver toxicity.

References

Sheikh A, Yeasmin F, Agarwal S, Rahman M, Islam K, Hossain E, et al. Protective effects of Moringa oleifera Lam. leaves against arsenic- induced toxicity in mice. Asian Pac J Trop Biomed. 2014;4:S353-8.

Stohs SJ and Hartman MJ. Review of the safety and efficiency of Moringa oleifera. Phytother Res. 2015;29(6):796-804.

Karthivashan G, Arulselvan P, Alimon AR, Safinar Ismail I, Fakurazi S. Competing role of bioactive constituents in Moringa oleifera extract and conventional nutrition on the performance of Cobb 500 Broilers. Biomed Res Int. 2015;2015: 970398.

Dolla S, Abdulkarim SM, Ahmad SH, Khoramnia A, Ghazali HM. Physicochemical properties and potential food applications of Moringa Oleifera Seed Oil blended with other vegetable oils. J Oleo Sci. 2014;63(8):811-22.

Jung IL. Soluble extract from Moringa oleifera leaves with a new anticancer activity. PLoS One. 2014;18:9(4) e95492.

Kasolo JN, Bimenya GS, Ojok L, Ochleng J, Ogwal-Okeng JW. Phyochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plant Res. 2010;4:753-7.

Zhang M, Swarts SG, Yin L, Liu C, Tian Y, Cao Y, Swarts M et al. Antioxidant properties of quercetin. Adv Exp Med Biol. 2011;915:283-9.

Bischoff SC. Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care. 2008;11:733-40.

Rivera L, Moron R, Sanchez M, Zarzuelo A, Galisteo M. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese Zucker rats. Obesity (Silver Spring). 2008;16: 2081-7.

Karthikesan K, Pari L, Menon VP. Antihyperlipidemic effect of chlorogenic acid and tetrahydro curcumin in rats subjected to diabetogenic agents. Chem Biol Interact. 2010;188:643-50.

Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review. Front Pharmacol. 2012;3:24.

Sreelatha S, Jeyachitra A, Padma PR. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food and Chemical Toxicology. 2011;49:1270-5.

Fahmy TA, Nahla SH, Rehab RA. Potential activity of Moringa Oleifera leaf extract and some active ingredients against diabetes in rats. International Journal of Scientific & Engineering Research.2015;6(5):1490-1500.

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990; 82(13):1107-12.

Doss A, Palaniswamy M, Angayarkanni T, Dhanabalan. Antidiabetic activity of water extract of Sollanum Trilobatum in alloxan- induced diabetes in rats. African journal of biotechnology. 2009; 8(20):5562-64.

Gupta R, Mathor M, Bajaj VK, Katariya P, Xadu S, Kamal R, et al. Evaluation of diabetic activity of Moringa oleifera in experimental diabetes. J Diabetes. 2012;4(2):164-71.

Liang W, Luo Z, Ge S, Li M, Du J, Yang M. et al. Oral administration of quercetin inhibits bone loss in rat model of diabetic osteopenia. European journal of pharmacology. 2011;(670):317-24.

Soltesz Z, Wanecq E, Lomba A, Portillo M, Pellati F, Szoko E, et al. Chronic benzylamine administration in the drinking water improves glucose tolerance reduce body weight gain and circulating cholesterol in high fat diet-fed mice. Pharmacological research. 2010;61:335-63.

Hunyadi A, Martins A, Hsieh T, Seres A, Zupko I. Chlorogenic acid and rutin play a major role in the in-vivo antidiabetic activity of morusalba leaf extract on type II diabetic rats. PLOS ONE. 2012;7(11):e50619.

King EJ, Armstrong AR. In: Practical clinical biochemistry CBS Publishers, Calcium, phosphorus and phosphate. New Delhi, India, 1988:45-8.

Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976;72:248-54.

Bancroft JD, Stevens A. In: Theory and practice of histological techniques.The haematoxylin and eosin. Churchill Livingstone, London, New York & Tokyo. 4th ed, Ch 6, 1996:99–112.

Cutillas N, Yellol GS, de Haro C, Vicente C, Rodríguez V, Ruiz J. Anticancer cyclometalated complexes of platinum group metals and gold. Coordination Chemistry Reviews. 2013;257(19-20):2784-97.

Verma VK, Singh N, Saxena P, Singh R. Anti-ulcer and antioxidant activity of Moringa Oleifera (Lam) leaves against aspirin and ethanol induced gastric ulcer in rats. Int Res J of Pharmaceuticals. 2012;2(2):46-57.

Park EJ, Cheenpracha S, Chang LC, Kondratyuk TP. Inhibition of lipopolysaccharide-induced cyclooxygenase-2 and inducible nitric oxide synthase expression by 4-[(2’-O-acetyl-alpha-L-rhamnosyloxy)benzyl] isothiocyanate from Moringa oleifera. Nutr Cancer. 2011;63:971-82.

Sangkitikomol W, Rocejanasaroj A, Tencomnao T. Effect of Moringa Oleifera on advanced glycation end- product formation and lipid metabolism gene expression in HepG2 cells. Genet Mol Res. 2014;13(1):723-35.

Williams RJ, Spencer JP, Rice-Evans C. Flavonoids: antioxidants or signalling molecules? Biol Med. 2004;36:838-49.

Kong AN, Yu R, Chen C, Mandlekar S. Signal transduction events elicited by natural products: role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch Pharm. Res. 2000;23:1-16.

Granado-Serrano AB, Martin MA, Bravo L, Goya L et al. Quercetin induces apoptosis via caspase activation, regulation of Bcl-2, and inhibition of PI-3-kinase/Akt and ERK pathways in a human hepatoma cell line (HepG2). J Nutr. 2006;136:2715-21.

Beatty ER, O’Reilly JD, England TG, McAnlis GT, Young IS, Geissler CA, et al. Effect of dietary quercetin on oxidative DNA damage in healthy human subjects. Br J Nutr. 2000;84(6):919-25.

Niering P, Michels G, Watjen W, Ohler S, Steffan B, Chovolou Y, et al. Protective and detrimental effects of kaempferol in rat H4IIE cells: implication of oxidative stress and apoptosis. Toxicol. Appl Pharmacol. 2005;209(2):114-22.

Berkovich L, Earon G, Ron I, Rimmon A, Vexler A, Lev-Ari S. Moringa Oleifera aqueous leaf extract down-regulates nuclear factor-kappa B and increases cytotoxic effect of chemotherapy in pancreatic cancer cells. BMC Complementary and Altern Med. 2013;13:212-18.

Wilson RD, Islam MS. Effects of white mulberry (Morus alba) leaf tea investigated in a type 2 diabetes model of rats. Acta Pol Pharm. 2015;72(1):153-60.

Guthrow CE, Morris MA, Day JF, Thorpe SR, Baynes JW. Enhanced nonenzymatic glucosylation of human serum albumin in diabetes mellitus. Proc Natl Acad Sci USA. 1979;(76):4258-61.

Charoensin S. Antioxidant and anticancer activities of Moringa oleifera leaves. Journal of Medicinal Plant Research. 2014;8(7):318-25.

Gupta AK, Misra N. Hepatoprotective Activity of Aqueous Ethanolic Extract of Chamomile capitula in paracetamol intoxicated albino rats. Am J Pharm Toxicol. 2006;(1):17-20.

Buraimoh AA, Bako G, Ibrahim FB. Hepatoprotective Effect of Ethanolic Leave Extract of Moringa oleifera on the Histology of Paracetamol Induced Liver Damage in Wistar Rats. International Journal of Animal and Veterinary Advances. 2011;3(1):10-3.

Koriem KM, Soliman RE. Chlorogenic and Caftaric Acids in Liver Toxicity and Oxidative Stress Induced by Methamphetamine. Journal of Toxicology. 2014;2014:583494.

Moridani MY, Pourahmad J, Bui H, Siraki A. O’Brien Dietary flavonoid iron complexes as cytoprotective superoxide radical scavengers Free Radical Biol Med. 2003;34:243-53.

Ravichandran RM, Rajendran D, Devapiriam M. Antioxidant study of quercetin and their metal complex and determination of stability constant by spectrophotometry method. Food Chem. 2014;146:472-8.

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Published

2016-12-28

How to Cite

Ali, F. T., Hassan, N. S., & Abdrabou, R. R. (2016). Hepatoprotective and antiproliferative activity of moringinine, chlorogenic acid and quercetin. International Journal of Research in Medical Sciences, 4(4), 1147–1153. https://doi.org/10.18203/2320-6012.ijrms20160799

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Original Research Articles