DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20201068

Decerebration induced by surgical transection of cerebral ganglion of crayfish

Baltazar Barrera Mera, Emilio Pérez Ortega, Rodrigo Banegas Ruiz, Yuri Jiménez Caprielova, Francisco Fabián Gómez Mendoza, Rodrigo A. Mendoza Aceves, Alan I. Valderrama Treviño

Abstract


Background: Since the neural structures of the crayfish brain closely resemble their equivalent in the mammals. This can be suggested by observing the similarity that exists in the brain divided by the surgical transection of the crayfish brain in which the protocerebrum remains attached to the first two cranial nerves, findings also described by Frederic Bremer in 1935 in cats with cerebral transection.

Methods: Total 11 Adult male crayfish were trained to respond with defense reflex, the animals were placed in water at 0°C, remained without any movement, and subsequently through a small incision of 3 mm in diameter in the medial antero region and dorsal cephalothorax region, a surgical section of the cerebral ganglion was performed. Immediately after surgery, metal microelectrodes were implanted to collect the activity of the photoreceptors and visual fibers.

Results: Once the defense reflex begins to recover in previously decerebrated crayfish, it means that it shows signs of reconnection. The isolated protocerebrum with the deutocerebrum olfactory lobe remain alive for several days and the neuronal connections were reestablished, as measured throughout the bilateral defense activity. The defense reflex was observed in all animals and then recovered after surgery.

Conclusions: The crayfish is an excellent model to work the visual activity, all coding of visual information was suppressed in de-cerebrated crayfish. The recovery of the neural disconnection is observed from 40 days, where the defence reflex appears again before visual stimuli.

 


Keywords


Crayfish, Crayfish brain, Decerebration, Defence reflex, Neuronal reconnection, Protocerebral disconnection

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References


Davis K, Huber R. Activity patterns, behavioural repertoires, and agonistic interactions of crayfish: a non-manipulative field study. Behaviour. 2007 Jan 1;144(2):229-47.

Fujisawa K, Takahata M. Physiological changes of premotor nonspiking interneurons in the central compensation of eyestalk posture following unilateral sensory ablation in crayfish. J Compar Physiol. 2007 Jan 1;193(1):127-40.

Liden WH, Phillips ML, Herberholz J. Neural control of behavioural choice in juvenile crayfish. Proceed Royal Society B: Biol Sci. 2010 Nov 22;277(1699):3493-500.

Mellon D, Lorton ED. Reflex actions of the functional divisions in the crayfish oculomotor system. J Compar Physiol. 1977 Jan 1;121(3):367-80.

Puche J, Barrera-Calva E, Barrera-Mera B. Protocerebral deafferentation effects on crayfish glycemic response: a protocerebral circadian pacemaker regulates the hemolymph sugar concentration. Rev Espan De Fisiol. 1993 Sep;49(3):151-5.

Barrera-Mera B, Block GD. Protocerebral circadian pacemakers in crayfish: evidence for mutually coupled pacemakers. Brain Res. 1990 Jul 9;522(2):241-5.

Bremer F. Cerveau" isole" et physiologie du sommeil. CR Soc Biol (Paris). 1935;118:1235-41.

Barrera-Mera B, Cibrian-Tovar J, García-Díaz DE. The role of protocerebrum in the modulation of circadian rhythmicity in the crayfish visual system. Brain Res Bullet. 1980 Nov 1;5(6):667-72.

Naka K, Kuwabara M. Two components from the compound eye of the crayfish. J Experim Biol. 1959 Mar 1;36(1):51-61.

Aréchiga H. Fuentes-Pardo B, Barrera-Mera B. Influence of retinal shielding pigments on light sensitivity in the crayfish. Acta Physiol Lat Am. 1974;24(6):601-11.

Bovbjerg RV. Dominance order in the crayfish Orconectes virilis (Hagen). Physiol Zoolog. 1953 Apr 1;26(2):173-8.