DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20202898

MALDI-TOF MS: the proteomic approach, the future of fungal identification in India

Mallika Fonseca, Sarala Menon, Praveen Rahi, Prachi Patekar, Abhay S. Chowdhary

Abstract


Background: India, being a country where fungal infections are rampant, is urgently in need of effective tools for early and accurate diagnosis of fungal infections. Matrix-assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) is a recent method which has shown potential in identifying clinically important bacterial pathogens as well as clinically important fungi. The main objective of this study was to compare the utility of MALDI-TOF MS for the identification of fungi against that of conventional methods.

Methods: The project was carried out in a tertiary care government hospital in India. Fifty clinical isolates comprising mainly various yeast species were subjected to conventional identification (Phenotypic) as well as MALDI-TOF-MS. Their results were further compared.

Results: MALDI-TOF MS showed a high concordance with conventional methods while identifying species like C. albicans, C. tropicalis, C. parapsilosis and C. neoformans, although the concordance for species such as Rhodotorula and Trichosporon could only be matched up to genus level.

Conclusions: MALDI-TOF MS-based identification is both a rapid and a viable tool for identification of clinically relevant yeast species with good correlation to conventional methods and a quick turnaround time.


Keywords


Mycoses diagnosis, Mycological typing techniques, Molecular diagnostic techniques, Mass spectrometry, Matrix assisted laser desorption-ionization

Full Text:

PDF

References


Verma S, Madhu R. The Great Indian Epidemic of Superficial Dermatophytosis: An Appraisal. Indian J Dermatol. 2017;62(3):227-36.

Panda A, Ghosh AK, Mirdha BR, Xess I, Paul S, Samantaray JC, et al. MALDI-TOF mass spectrometry for rapid identification of clinical fungal isolates based on ribosomal protein biomarkers. J Microbiol Methods. 2015;109:93-105.

González de Molina FJ, León C, Ruiz-Santana S, Saavedra P. Assessment of candidemia-attributable mortality in critically ill patients using propensity score matching analysis. Critical Care. 2012;16(3):R105.

Guery BP, Arendrup MC, Auzinger G. Management of invasive candidiasis and candidemia in adult non-neutropenic intensive care unit patients: part I. Epidemiology and diagnosis. Intensive Care Med. 2009;35(1):55-62.

Chalupová J, Raus M, Sedlářová M, Sebela M. Identification of fungal microorganisms by MALDI-TOF mass spectrometry. Biotechnol Adv. 2014 Jan-Feb;32(1):230-41.

Cassagne C, Ranque S, Normand AC, Fourquet P, Thiebault S, Planard C, et al. Mould Routine Identification in the Clinical Laboratory by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry. PLoS ONE 2011;6(12):e28425.

Baron EJ, Finegold, SM. Baily and Scott’s Diagnostic Microbiology. 8th Edition. St. Louis, C.V. Mosby Company; 1990:7.

Koneman’s colour atlas and Textbook of Diagnostic Microbiology Practical. 6th Ed.1997.

Murray PR. Manual of Clinical Microbiology. 8th Ed. Washington, D.C: ASM Press.

Sutton DA, Fothergill AW, Rinaldi MG. Guide to Clinically Significant Fungi. Williams and Wilkins, 1998.

Fenselau C, Demirev PA. Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrom Re. 2001;20:157-71.

Rahi P, Sharma, OP, Shouche YS. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists. Frontiers Microbiol. 2016;7:1359.

Ryzhov V, Fenselau C. Characterization of the protein subset desorbed by MALDI from whole bacterial cells. Anal Chem. 2001;73:746-50.

Suarez S, Ferroni A, Lotz A, Jolley KA, Guérin P, Leto J, et al. Ribosomal proteins as biomarkers for bacterial identification by mass spectrometry in the clinical microbiology laboratory. J Microbiol Methods. 2013;94(3):390-6.

Croxatto A, Prod’hom G, Greub G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiol Rev. 2012;36:380-407.

Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL. Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011 Apr;49(4):1614-6.

Spanu T, Posteraro B, Fiori B. Direct MALDI-TOF Mass Spectrometry Assay of Blood Culture Broths for Rapid Identification of Candida Species Causing Bloodstream Infections: an Observational Study in Two Large Microbiology Laboratories. J Clini Microbiol. 2012;50(1):176-9.