DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20204237

Estimation of interleukin-17 levels in gingival crevicular fluid from healthy individuals, chronic gingivitis and chronic periodontitis patients using enzyme linked immunosorbent assay

Neha Nainee, Sheetal Sanikop, Abhilasha Jha

Abstract


Background: Chronic Periodontitis (CP) is an infectious disease resulting in inflammation of supporting tissues of the teeth. A number of pro-inflammatory cytokines are formed against periodontopathogenic microorganisms. Interleukin-17 (IL-17) is a pro-inflammatory cytokine, implicated in numerous inflammatory and autoimmune conditions.

Methods: A total of 25 periodontally healthy subjects (Group 1), 25 patients with gingivitis (Group 2) and 25 patients with CP (Group 3) were included for the study based on clinical examination. Gingival index, probing pocket depth and clinical attachment loss were recorded in all subjects.

Results: The levels of IL-17 increased from healthy to gingivitis to periodontitis patients. A positive correlation was found with the IL-17 and the clinical parameters like gingival index, probing pocket depth and clinical attachment loss.

Conclusions: There is a strong association between the levels of IL-17 with periodontal disease as well as with its severity and its possible use as a biomarker for inflammatory periodontal disease.


Keywords


Periodontitis, Gingival crevicular fluid, Enzyme linked immunosorbent assay, IL-17

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References


Newman MG, Takei HH, Klokkevold PR, Carranza FA. Carranza’s Clinical Periodontology, 10th e. St. Louis, Missouri: Saunders, Elsevier,2006.

Takahashi K, Azuma T, Motohira H, Kinane DF, Kitetsu S. The potential role of interleukin-17 in the immunopathology of periodontal disease. J Clin Periodontol 2005; 32: 369–374.

3Jill M. Kramer, Sarah L. Gaffen. Interleukin-17: A New Paradigm in Inflammation, Autoimmunity, and Therapy. J Periodontal 2007;78(6):1083-1093.

Mangan PR, Harrington LE, O’Quinn DB, et al. Transforming growth factor-beta induces development of the T(H)17 lineage. Nature 2006;441:231-234.

Bettelli E, Carrier Y, Gao W, et al. Reciprocal developmental pathways for the generation of pathogenic effector T(H)17 and regulatory T cells. Nature 2006;441:235-238.

Vernal R, Dutzan N, Chaparro A, Puente J, Antonieta Valenzuela M, Gamonal J. Levels of interleukin-17 in gingival crevicular fluid and in supernatants of cellular cultures of gingival tissue from patients with chronic periodontitis. J Clin Periodontol 2005;32:383-389.

Theill L, Boyle W, Penninger J. RANK-L and RANK: T cells, bone loss and mammalian evolution. Annu Rev Immunol 2002;20:795-823.

Kotake S, Udagawa N, Takahashi N, et al. IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest 1999;103:1345-1352.

Teitelbaum SL, Ross FP. Genetic regulation of osteoclast development and function. Nat Rev Genet 2003;4:638-649.

Roodman GD. Regulation of osteoclast differentiation. Ann N Y Acad Sci 2006;1068:100-109.

Taubman MA, Valverde P, Han X, Kawai T. Immune response: The key to bone resorption in periodontal disease. J Periodontol 2005;76:2033-2041.

Ravindra Reddy Nagireddy, Vinay Chavan, Madhu Babu Dandu Subramanyam, Venkat Subba Chowdavaram Reddy, Mohan Kumar Pasupuleti, Kishore Kumar Avula. Estimation of Interleukin 17 levels in gingival crevicular fluid from healthy individuals and patients with chronic periodontitis. J NTR Univ Health Sci 2013:2(3):191-195.

Akio Mitani, Wanda Niedbala, Takeki Fuzimura, Makio Mogi, Shin Miyamae, Naoya Higuchi et al. Increased expression of IL-35 and IL-17, but not IL-27, in gingival tissues with chronic periodontitis. J Periodontal 2015;86:301-309.

Erry Mochamad Arief, Marina Binti Mubin, Siti Lailatul Akmar Zainuddin, Nurul Asma Abdullah, Basaruddin Ahmad. Serum interleukin-17 (IL-17) in chronic periodontitis patients. Padjadjaran J Dent 2017;29(3):138-142.

Husniah Batool, Ahmed Nadeem, Muhammad Kashif, Faheem Shahzad, Romeeza Tahir, Nadeem Afzal. Salivary levels of IL-6 and IL-17 could be an indicator of disease severity in patients with calculus associated chronic periodontitis. BioMed Res Int 2018

Lamster IB, Novak MJ. Host mediators in gingival crevicelar fluid:implications for pathogenesis of periodontal disease. Crit Rev Oral Biol Med 1992;3:31-60

Nedzi-Gora M, Kostrzewa-Janicka J, Gorska R. Markers of inflammation in periodontal diseases. Cent Eur J Immunol 2013;38:363-366.

Khader SA, Gaffen SL, Kolls JK. TH17 cells at the crossroads of innate and adaptive immunity against infectious disease at the mucosa. Mucosal Immunol 2009;2:403-411.

Azman R, Lappin DF, MacPherson A, Robertson D, Hodge P, Ramage G et al. Clinical association between IL-17 family cytokines and periodontitis and potential differential roles for IL-17A and IL-17E in periodontal immunity.Inflammation Res 2014;63(12):1001-1012.