Comparison of direct total iron binding capacity by light MgCO3 and heavy MgCO3

Chapal Debnath, Niral G. Savaliya


Background: The aim of the study is focused to measure TIBC by calculated method (TIBC using the magnesium carbonate- MgCO3 adsorption), measurement of TIBC by calculatory method from automated UIBC and automated Iron measurement. Latter these two values are to be added and would give an additive result of TIBC. The experiment would be performed by using the light MgCO3 powder and the heavy MgCO3 powder and check for any differences in their respective values. Regression analysis, Bland Altman analysis or histogram analysis of TIBC results obtained by calculated methods in patient samples to find correlation between the two methods is being performed.

Methods: Samples received for analysis of tests, regardless of patient identification with sample volume serum were used for the study. After complete analysis and reporting of the sample, the leftover serum was used. Serum was kept at room temperature. Then the calculated TIBC from UIBC and the IRON were compared after doing batch calibration for UIBC and lot calibration of Iron.

Results: It was found that, this study had positive bias by the usage of the heavy MgCO3 powder. Positive bias in unsaturated iron binding capacity observed by both methods is studied and further investigated using data obtained during the experiment.

Conclusions: There is a higher yield of serum obtained with the heavy MgCO3 powder, TIBC calculated from iron and unsaturated iron binding capacity as compared to TIBC measured directly using the light MgCO3 powder.


Iron, UIBC, TIBC, MgCO3, Calculated method, Automatic analyser

Full Text:



Frazer DM, Anderson GJ. Iron imports. I. Intestinal iron absorption and its regulation. Am J Physiol Gastrointest Liver Physiol. 2005;289:G631-5.

Nadadur SS, Srirama K, Mudipalli A. Iron transport and homeostasis mechanisms: Their role in health and disease. Indian J Med Res. 2008;128:533-44.

Yeh KY, Yeh M, Mims L, Glass J. Iron feeding induces ferroportin 1 and hephaestin migration and interaction in rat duodenal epithelium. Am J Physiol Gastrointest Liver Physiol. 2009;296:55-65.

Theil EC, Chen H, Miranda C, Janser H, Elsenhans B, Núñez MT, et al. Absorption of iron from ferritin is independent of heme iron and ferrous salts in women and rat intestinal segments. J Nutr. 2012; 142:478-83.

Finberg KE. Unraveling mechanisms regulating systemic iron homeostasis. Am Soc Hematol. 2011; 1:532-7.

Aisen P, Enns C, Wessling-Resnick M. Chemistry and biology of eukaryotic iron metabolism. Int J Biochem Cell Biol. 2001;33:940-59.

Lieu PT, Heiskala M, Peterson PA, Yang Y. The roles of iron in health and disease. Mol Aspects Med. 2001;2:1-87.

Hurrell R, Egli I. Iron bioavailability and dietary reference values. Am J Clin Nutr. 2010;91:1461-7S.

Satyanarayana UC. Mineral metabolism. 3rd ed. Netherlands: Elsevier; 2006.

Quintero-Gutiérrez AG, González-Rosendo G, Sánchez-Muñoz J, Polo-Pozo J, Rodríguez-Jerez JJ. Bioavailability of heme iron in biscuit filling using piglets as an animal model for humans. Int J Biol Sci. 2008;4:58-62.

Bobby JC. The role of iron in the immune response to bacterial infection. Immunol Res. 2011;50(1):1-9.

McDowell LR. Vitamins in animal and human nutrition, 2nd ed. USA: Iowa State University Press; 2000.

Richard FH, Davidsson L, Reddy M, Kastenmayer P. A comparison of iron absorption in adults and infants consuming identical infant formulas. Br J Nutr. 1998;79:31-6.

Institute of medicine, panel on micronutrients. Dietary reference intakes for vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. Washington (DC): National Academies Press (US); 2001.

Muir A, Hopfer U. Regional specificity of iron uptake by small intestinal brush-border membranes from normal and iron deficient mice. Am J Physio. 1985;248:G376-9.

Blanck HM, Pfeiffer CM, Caudill SP, Reyes M, Gunter EW, Imperatore G, et al. Serum iron and iron-binding capacity: a round-robin interlaboratory comparison study. Clin Chem. 2003;49:1672-5.

Melanson SE, Lindeman NI, Jarolim P. Selecting automation for the clinical chemistry laboratory BRENDSTRUPP: Serum iron, total iron-binding capacity of serum, and serum copper in normals. Arch Patho Lab Med. 2007;131:1063-9.

Fairbanks VF, Klee GG. Biochemical aspects of hematology. In: Burtis CA, Ashwood ER, eds. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia: WB Saunders; 1994:1974-2072.

Huebers HA, Eng MJ, Josephson BM, Ekpoom N, Rettmer RL, Labbé RF, et al. Plasma iron and transferrin iron-binding capacity evaluated by colorimetric and immunoprecipitation methods. Clin Chem. 1987;33:273-7.

Gale E, Torrance J, Bothwell T. The quantitative estimation of total iron stores in human bone marrow. J Clin Invest. 1963;42(7):1076-82.

Hiroshi S. New methods for determining the total iron-binding capacity of serum. J Nucl Med. 1971; 12:489-92.

Malletr BB. The determination of iron in plasma or serum. Biochem. 1955;59:599-602.

Stojceski TK, Malpas JS, Witts LJ. Studies on the serum iron-binding capacity. J Clin Pathol. 1965; 18(4):446-52.

Bothwell TH, Jacob SP, Kamene RR. Determination of unsaturated iron-binding capacity of serum using radioactive iron. J Clin Pathol. 1965;18(4): 453-5.

Cartwright GE, Wintrobe MM. Chemical, dm1 cal and immunological studies on products of human plasma fractionation. J Clin Invest. 1949; 28(1):86-98.